Novel Alleles 8 and 9 Strains of Mycoplasma mycoides subsp. mycoides circulating in South East Nigeria and Comparison with Vaccine Reference Strain T1/44

Control of contagious bovine pleuropneumonia (CBPP) is difficult in Nigeria due to lack sufficient data. Mycoplasma mycoides subsp. the causative organism CBPP. This disease endemic with serious economic impact. study was undertaken characterize M. (Mmm) cattle south-east using single-locus sequence analysis based on polymorphism non-coding genes and also comparison obtained sequences vaccine reference strain (T1/44) that presently used Nigeria. Twenty one Polymerase chain reaction (PCR) confirmed Mmm isolates from Anambra Enugu states were for this study. The amplicons amplified locus PG1 (LocPG1-0001) sequenced corresponding primers. edited BioEdittm software a consensus obtained. Thereafter, aligned ClustalW (BioEdittm). compared allele numbers assigned any nucleotide changes. Three allelic obtained; Allele 1, 8 9.  number 9 are new findings as they not previously reported Points observed between field isolates. 21 deposited Genbank following accession numbers; MW487814-MW487834.     Key words: Alleles, multi-locus analysis, mycoides, Nigeria, polymorphism.